Travel Republic took the top spot in The Sunday Times Virgin Atlantic Fast Track 100 with an annual sales growth of 284.2% having clocked up sales £14.6 million in the 2006/07 financial year.
TravelRepublic has also been named as the fastest growing non-user generated content online brand in the UK by Nielsen NetRatings, increasing its unique audience by 313% between October 2006 and October 2007.
NetRatings said the OTA secured an audience of 557,000 in October 2007.
The only UK sites to have grown quicker than TravelRepublic in the past 12 months include Veoh, Hot or Not, Slide, Facebook and RockYou!.
Managing director Paul Furner said the company, which employs more than 150 staff in south west London is predicted to generate a turnover of £200 million for this year.
He added: “At a time when it is often said that there is no loyalty on the web we would beg to differ. Our levels of repeat and recommended business suggest that we have a real affinity with our customers which we plan to build upon in the coming months.”
Meanwhile online bed bank Alpharooms came 20th in the survey having generated growth of 121.2% from sales of £5.5 million.
Managing director Jason Fry said: “The growth of the company has seen us strengthen our team in Sheffield from a staff of just 20 in 2004 to more than 80 people, and this accolade is testament to the hard work of the entire team.”